Journal: International Journal of Molecular Medicine

Article Title: Rab27: Molecular switch of tumor exosome secretion (Review)

doi: 10.3892/ijmm.2026.5853

Figure Lengend Snippet: Structural characteristics of Rab27a and Rab27b. (A) Sequence alignment of the region between the Rab27a and Rab27b domains. Identical amino acids are highlighted with a red background and white font; functionally similar amino acids are indicated with a white background and red font; and divergent amino acids are shown in black font. (B) Post-translational modification sites of Rab27a and Rab27b.

Article Snippet: Irep et al , 2024 , Small cell lung cancer , Rab27a , Nexinhib20 , Exosome secretion , Preclinical stage , ( ) .

Techniques: Sequencing, Modification

Journal: International Journal of Molecular Medicine

Article Title: Rab27: Molecular switch of tumor exosome secretion (Review)

doi: 10.3892/ijmm.2026.5853

Figure Lengend Snippet: Rab switch form and subcellular localization of Rab27. Rab proteins exist in two reversible states: The inactive GDP-bound form and the active GTP-bound form. In the inactive state, Rab is bound to GDP. GEFs catalyze the exchange of GDP with GTP, thereby activating Rab. The GTP-bound active form of Rab plays a crucial role in vesicular transport. Subsequently, GAPs promote the hydrolysis of GTP to GDP, returning Rab to its inactive state. Inactive GDP-bound Rab is then recognized by the REP, and in the presence of geranyl-geranyl-transferase, it associates with GDIs and GDFs, which regulate the membrane cycling of Rab proteins. Rab27a is localized to melanosomes, secretory granules, late endosomes and lysosomes. Rab27b is predominantly found in the membrane of Golgi stacks and in vesicles located in the TGN area, secretory granules, and late endosomes. GEFs, guanine nucleotide exchange factors; GAPs, GTPase-activating proteins; GDIs, guanine nucleotide dissociation inhibitors; REP, Rab escort protein; GDFs, GDP dissociation stimulator factors; TGN, trans-Golgi network; ER, endoplasmic reticulum; MVBs, multivesicular bodies.

Article Snippet: Irep et al , 2024 , Small cell lung cancer , Rab27a , Nexinhib20 , Exosome secretion , Preclinical stage , ( ) .

Techniques: Membrane

Journal: bioRxiv

Article Title: Extracellular Vesicles from Senescent Tumor Cells Are Necessary and Sufficient to Drive Paracrine Senescence

doi: 10.64898/2026.03.25.713920

Figure Lengend Snippet: (a) RT-qPCR analysis of RAB27A . GAPDH was used as housekeeping gene. (b) Western blot analysis of RAB27A. Vinculin was used as loading control. (c) NTA analysis of particles in CM. (d) Representative images of morphology and SA-β-Gal staining (scale, 100 μm; scale in zoomed area, 200 μm). (e) Cell counting. (f) Cell size measurement. (g) Quantification of SA-β-Gal positive cells using X-gal substrate. (h) Quantification of SA-β-Gal positive cells using C 12 FDG substrate. (i) RT-qPCR analysis of CDKN1A . GAPDH was used as housekeeping gene. Significance was calculated using one-way ANOVA showing the exact p-value. Less than 0.05 was considered statistically significant (a, c-i, n=3; b, n=1).

Article Snippet: Membranes were blocked with 5% milk solution and incubated with primary antibodies for GAPDH (sc-32233, Santa Cruz Biotechnology 1:1000), β-Actin (sc-8432, Santa Cruz Biotechnology, 1:1000), Vinculin (sc-76314, Santa Cruz Biotechnology 1:2000), RAB27A (#69295, Cell Signaling Technology, 1:1000), p53 (sc-6243, Santa Cruz Biotechnology, 1:1000), and p21 (#2947, Cell Signaling Technology, 1:1000), overnight at 4 °C.

Techniques: Quantitative RT-PCR, Western Blot, Control, Staining, Cell Counting

Journal: bioRxiv

Article Title: Extracellular Vesicles from Senescent Tumor Cells Are Necessary and Sufficient to Drive Paracrine Senescence

doi: 10.64898/2026.03.25.713920

Figure Lengend Snippet: (a) Representative images of morphology and SA-β-GAL staining (scale, 100 μm; scale in zoomed area, 200 μm). (b) Quantification of SA-β-GAL positive cells using X-gal substrate. (c) Flow cytometry plots of cells treated with conditioned media derived from control (CM-Scr), senescent (CM-Scr-S), or GW4869-treated senescent (CM-Scr-S+GW4869) cells, all of them expressing control Scrambled shRNA, and from RAB27A shRNA knockdown senescent cells (CM-shRAB27A #313-S). Significance was calculated using one-way ANOVA. Exact p-values are shown. Less than 0.05 was considered statistically significant (n=3).

Article Snippet: Membranes were blocked with 5% milk solution and incubated with primary antibodies for GAPDH (sc-32233, Santa Cruz Biotechnology 1:1000), β-Actin (sc-8432, Santa Cruz Biotechnology, 1:1000), Vinculin (sc-76314, Santa Cruz Biotechnology 1:2000), RAB27A (#69295, Cell Signaling Technology, 1:1000), p53 (sc-6243, Santa Cruz Biotechnology, 1:1000), and p21 (#2947, Cell Signaling Technology, 1:1000), overnight at 4 °C.

Techniques: Staining, Flow Cytometry, Derivative Assay, Control, Expressing, shRNA, Knockdown